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Atividade da alfavaca-cravo no biofilme microbiano da bolsa periodontal – estudo-piloto

Estudo avalia o efeito da alfavaca-cravo sobre os microrganismos orais obtidos da bolsa periodontal, comparando-a com o digluconato de clorexidina.


AUTORES

Erika Teles de Vasconcelos
Cirurgiã-dentista – Universidade de Fortaleza.
Orcid: 0000-0003-1280-7701.

Julyhara de Pinho Gomes
Cirurgiã-dentista – Universidade de Fortaleza.

Priscila Almeida Lins Menezes
Cirurgiã-dentista – Universidade de Fortaleza.

Priscilla Maria Albuquerque Oliveira
Cirurgiã-dentista – Universidade de Fortaleza.

Thaís Cavalcante Rabelo
Cirurgiã-dentista – Universidade de Fortaleza.

Camila de Brito Pontes
Cirurgiã-dentista – Universidade de Fortaleza.
Orcid: 0000-0001-7443-677X.

Márcia Maria Negreiros Pinto Rocha
Professora do curso de Odontologia – Universidade de Fortaleza.
Orcid: 0000-0001-9818-3316.

Sérgio Luís da Silva Pereira
Professor do curso de Odontologia – Universidade de Fortaleza.
Orcid: 0000-0003-0309-5159.

Flávio Nogueira da Costa
Professor do curso de Farmácia – Centro Universitário Católico.
Orcid: 0000-0002-7034-332x.

RESUMO

Objetivo: avaliar o efeito da alfavaca-cravo sobre os microrganismos orais obtidos da bolsa periodontal, comparando- a com o digluconato de clorexidina. Material e métodos: os microrganismos testados pertenciam ao biofilme de uma bolsa periodontal de 6 mm do canino superior direito, obtido de um voluntário normossêmico com periodontite crônica não tratada. A atividade antibacteriana foi determinada pelo método de difusão em ágar. Os microrganismos foram imersos em uma solução de BHI (brain heart infusion) e distribuídos em placas contendo ágar-sangue em contato com as seguintes substâncias: salina a 0,9%; digluconato de clorexidina a 0,12% (gel e solução); alfavaca-cravo a 20% e 40% (extrato hidroalcoólico e gel). Resultados: a solução salina não teve efeito sobre o crescimento bacteriano, semelhante à alfavaca-cravo. O digluconato de clorexidina a 0,12% (gel e solução) demonstrou um expressivo halo de inibição. Conclusão: a alfavaca-cravo não demonstrou efeito antimicrobiano in vitro sobre amostras microbiológicas de biofilme da bolsa periodontal.

Palavras-chave – Microbiologia; Medicina herbal; Doenças periodontais.

ABSTRACT

Objective: to evaluate the effect of the Ocimum gratissimum on oral microorganisms from periodontal pocket, compared to chlorhexidine digluconate. Material and methods: antibacterial activity was determined by agar diffusion. The microorganisms were immerged in a pre-reduced BHI (brain heart infusion) and distributed in plates containing agar-sanguis in contact with various solutions: 0.9% saline; 0.12% chlorhexidine digluconate (gel and solution); 20% and 40% Ocimum gratissimum (hydroalcoholic extract and gel). Results: the saline solution had no effect on bacterial growth in a similar manner than Ocimum gratissimum. 0.12% chlorhexidine digluconate (gel and solution) showed an expressive inhibition hale (13 mm). Conclusion: Ocimum gratissimum hydroalcoholic extract did not show inhibitory effect in vitro on microorganisms from subgingival biofilm.

Key words – Microbiology; Herbal medicine; Periodontal diseases.

Recebido em abr/2020
Aprovado em mai/2020

Referências

  1. Hartenbach FARR, Silva-Boghossian CM, Colombo APV. The effect of supragingival biofilm re-development on the subgingival microbiota in chronic periodontitis. Arch Oral Biol 2018;85:51-57.
  2. Ehizele A, Akhionbare O. Effect of non-surgical periodontal therapy on the concentration of volatile sulfur compound in mouth air of a group of nigerian young adults. Ann Med Health Sci Res 2013;3(3):433-7.
  3. Mohan R, Agrawal S, Gundappa M. Atomic force microscopy and scanning electron microscopy evaluation of efficacy of scaling and root planning using magnification: a randomized controlled clinical study. Contemp Clin Dent 2013;4(3):286-94.
  4. Jain M, Dave D, Jain P, Manohar B, Yadav B, Shetty N. Efficacy of xanthan based chlorhexidine gel as an adjunct to scaling and root planning in treatment of the chronic periodontitis. J Indian Soc Periodontol 2013;17(4):439-43.
  5. Jagadish Pai BS, Rajan SA, Srinivas M, Padma R, Suragimath G, Walvekar A et al. Comparison of the efficacy of chlorhexidine varnish and chip in the treatment of chronic periodontitis. Contemp Clin Dent 2013;4(2):156-61.
  6. Moghaddam A, Radafshar G, Jahandideh Y, Kakaei N. Clinical evaluation of effects of local application of aloe vera gel as an adjunct to scaling and root planning in patients with chronic periodontitis. J Dent (Shiraz) 2017;18(3):165-72.
  7. Thaweboon S, Thaweboon B. In vitro antimicrobial activity of Ocimum americanum L. essential oil against oral microorganisms. Southeast Asian J Trop Med Public Health 2009;40(5):1025-33.
  8. Saxena S, Lakshminarayan N, Gudji S, Kumar M. Antibacterial efficacy of terminalia chebula, terminalia bellirica, embilica officinalis and Triphala on salivary Streptococcus Mutans count – a linear randomized cross over trial. J Clin Diagn Res 2017;11(2):47-51.
  9. Ahonkai I, Ayinde BA, Edogun O, Um U. Antimicrobial activities of the volatile oils of Ocimum bacilicum L. and Ocimum gratissimum L. (Lamiaceae) against some aerobic dental isolates. Pak J Pharm Sci 2009;22(4):405-9.
  10. Sokovic M, Glamoclija J, Marin PD, Brkić D, van Griensven LJ. Antibacterial effects of the essential oils of commonly consumed medicinal herbs using an in vitro model. Molecules 2010;15(11):7532-46.
  11. Galindo LA, Pultrini AM, Costa M. Biological effects of Ocimum gratissimum L. are due to synergic action among multiple compounds present in essential oil. J Nat Med 2010;64(4):436-41.
  12. Pereira CAM, Maia JF. Study of antioxidant activity of extract and essential oil obtained from alfavaca leaves (Ocimum gratissimum L.). Food Sci Technol 2007;27(3):624-32.
  13. Aguiyi JC, Obi CI, Gang SS, Igweh AC. Hypoglycaemic activity of Ocimum gratissimum in rats. Fitoterapia 2000;71(4):444-6.
  14. Pereira SL, Oliveira JW, Angelo KK, Costa AMA, Costa F. Clinical effect of a mouth rinse containing Ocimum gratissimum on plaque and gingivitis control. J Contemp Dent Pract 2011;12(5):350-5.
  15. Pimenta MS, Lobo NS, Vieira VC, Costa AMA, Costa FN, Pereira SLS. Effect of Ocimum gratissimum in mouthrinses on de novo plaque formation. A randomized clinical trial. Braz Dent J 2016;27(6):646-51.
  16. Silva NL, Bezerra RA, Costa FN, Rocha MMNP, Pereira SLS. Evaluation of the activity of Anacardium occidentale on microorganisms of subgingival biofilm: experimental study in vitro. Braz J Periodontol 2013;23(4):26-30.
  17. Oliveira JDC, Pimentel JO, Macedo SRA, Mota OML, Rocha MMNP, Pereira SLS. Effect of iodine-povidone on subgingival biofilm microorganisms: an experimental study in vitro. Braz J Health Res 2009;11:4-8.
  18. Rocha ARA, Soares CL, Pereira SLS, Rocha MMNP. Effect of different chemical agents on microorganisms of supragingival biofilm: experimental study in vitro. Ufes Rev Odontol 2008;10:27-30.
  19. Ellepola ANB, Khan ZU, Chandy R, Philip L. A comparison of the antifungal activity of herbal toothpastes against other brands of toothpastes on clinical isolates of Candida albicans and Candida dubliniensis. Med Princ Pract 2011;20(2):112-7.
  20. Araújo CRF, Pereira JV, Pereira MSV, Alves PM, Higino JS, Martins AB. Bactericidal minimum concentration of cajueiro extract on bacteria from the biofilm dental. Pesq Bras Odontoped Clin Integr 2009;9:187-91.
  21. Nweze EI, Eze EE. Justification for the use of Ocimum gratissimum L in herbal medicine and its interaction with disc antibiotics. BMC Complement Altern Med 2009;9:1-6.
  22. Silva JG, Souza IA, Higino JS, Siqueira-Júnior JP, Pereira JV, Pereira MSV. Antimicrobial activity of hydroalcoholic Anacardium occidentale Linn extract against multi-drug resistant samples of Staphylococcus aureus. Rev Bras Farmacogn 2007;17(4):572-7.
  23. Laekeman GM, Hoof LV, Haemers A, Vanden Berghe DA, Herman AG, Vlietinck AJ. Eugenol a valuable compound for in vitro experimental research and worthwhile for further in vivo investigation. Phytoth Res 1990;4(3):90-6.
  24. Bairy I, Reeja S, Siddharth, Rao PS, Bath M, Shivananda PG. Evaluation of antibacterial activity of mangifera indica on anaerobic dental microflora based on in vivo studies. Indian J Pathol Microbiol 2002;45(3):307-10.
  25. Carvalho FG, Negrini TC, Sacramento LVS, Hebling J, Spolidorio DM, Duque C. The in vitro antimicrobial activity of natural infant fluoride-free toothpastes on oral micro-organisms. J Dent Child (Chic) 2011;78(1):3-8.
  26. Silva MRR, Oliveira Jr. JG, Fernandes OFL, Passos XS, Costa CR, Souza LKH et al. Antifungal activity of Ocimum gratissimum towards dermatophytes. Mycoses 2005;48(3):172-5.
  27. Lemos JA, Passos XS, Fernandes OFL, de Paula JR, Ferri PH, Hasimoto e Souza LK et al. Antifungal activity from Ocimum gratissimum L. towards Cryptococcus neoformans. Mem Inst Oswaldo Cruz 2005;100(1):55-8.
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