Atividade da alfavaca-cravo no biofilme microbiano da bolsa periodontal – estudo-piloto

Estudo avalia o efeito da alfavaca-cravo sobre os microrganismos orais obtidos da bolsa periodontal, comparando-a com o digluconato de clorexidina.

AUTORES

Erika Teles de Vasconcelos
Cirurgiã-dentista – Universidade de Fortaleza.
Orcid: 0000-0003-1280-7701.

Julyhara de Pinho Gomes
Cirurgiã-dentista – Universidade de Fortaleza.

Priscila Almeida Lins Menezes
Cirurgiã-dentista – Universidade de Fortaleza.

Priscilla Maria Albuquerque Oliveira
Cirurgiã-dentista – Universidade de Fortaleza.

Thaís Cavalcante Rabelo
Cirurgiã-dentista – Universidade de Fortaleza.

Camila de Brito Pontes
Cirurgiã-dentista – Universidade de Fortaleza.
Orcid: 0000-0001-7443-677X.

Márcia Maria Negreiros Pinto Rocha
Professora do curso de Odontologia – Universidade de Fortaleza.
Orcid: 0000-0001-9818-3316.

Sérgio Luís da Silva Pereira
Professor do curso de Odontologia – Universidade de Fortaleza.
Orcid: 0000-0003-0309-5159.

Flávio Nogueira da Costa
Professor do curso de Farmácia – Centro Universitário Católico.
Orcid: 0000-0002-7034-332x.

RESUMO

Objetivo: avaliar o efeito da alfavaca-cravo sobre os microrganismos orais obtidos da bolsa periodontal, comparando- a com o digluconato de clorexidina. Material e métodos: os microrganismos testados pertenciam ao biofilme de uma bolsa periodontal de 6 mm do canino superior direito, obtido de um voluntário normossêmico com periodontite crônica não tratada. A atividade antibacteriana foi determinada pelo método de difusão em ágar. Os microrganismos foram imersos em uma solução de BHI (brain heart infusion) e distribuídos em placas contendo ágar-sangue em contato com as seguintes substâncias: salina a 0,9%; digluconato de clorexidina a 0,12% (gel e solução); alfavaca-cravo a 20% e 40% (extrato hidroalcoólico e gel). Resultados: a solução salina não teve efeito sobre o crescimento bacteriano, semelhante à alfavaca-cravo. O digluconato de clorexidina a 0,12% (gel e solução) demonstrou um expressivo halo de inibição. Conclusão: a alfavaca-cravo não demonstrou efeito antimicrobiano in vitro sobre amostras microbiológicas de biofilme da bolsa periodontal.

Palavras-chave – Microbiologia; Medicina herbal; Doenças periodontais.

ABSTRACT

Objective: to evaluate the effect of the Ocimum gratissimum on oral microorganisms from periodontal pocket, compared to chlorhexidine digluconate. Material and methods: antibacterial activity was determined by agar diffusion. The microorganisms were immerged in a pre-reduced BHI (brain heart infusion) and distributed in plates containing agar-sanguis in contact with various solutions: 0.9% saline; 0.12% chlorhexidine digluconate (gel and solution); 20% and 40% Ocimum gratissimum (hydroalcoholic extract and gel). Results: the saline solution had no effect on bacterial growth in a similar manner than Ocimum gratissimum. 0.12% chlorhexidine digluconate (gel and solution) showed an expressive inhibition hale (13 mm). Conclusion: Ocimum gratissimum hydroalcoholic extract did not show inhibitory effect in vitro on microorganisms from subgingival biofilm.

Key words – Microbiology; Herbal medicine; Periodontal diseases.

Recebido em abr/2020
Aprovado em mai/2020

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